J.A. Callow, H.W. Woolhouse (Eds.)'s Advances in Botanical Research, Vol. 11 PDF

By J.A. Callow, H.W. Woolhouse (Eds.)

ISBN-10: 0120059118

ISBN-13: 9780120059119

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This instrument has been successfully calibrated against standard systems for diffusion and translational motion and used for biological investigations of sieve tubes (Johnson, 1983; Johnson and Ross, 1983). 4 . Future Designs It is apparent from the above descriptions that commercial light microscopes are far from suitable starting points for the construction of a laser Doppler micro- LASER LIGHT SCATTERlNG IN BIOLOGICAL RESEARCH 33 scope. They require extensive modification, but yet, while giving excellent viewing facilities for observing the specimen, they still fail to provide the necessary flexibility for the laser beam systems.

The light beam, which can be attenuated by neutral density filters, passes into the epiillumination system of the microscope. Both the microscope and the optical bench are isolated from building vibrations by being mounted on a heavy (270 kg) polished granite slab. The instruments are attached to the slab by double-sided adhesive tape to prevent accidental displacement. The slab is supported on three automobile inner tubes inflated just sufficiently to lift it from the bench. A mounting arrangement of this type is absolutely essential to provide protection from external vibrations.

Also the role of tropomyosin in reducing the flexibility of the actin filaments was confirmed. As a climax to these in vitro studies, cross-bridge cycling was studied under conditions of declining ATP availability. However, application of these methods to the in vivo situation was less satisfactory, possibly because the light scattering signals expected from the moving myosin molecules are masked by other dynamic events in the muscle, or perhaps because the models for such motions are imperfect.

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Advances in Botanical Research, Vol. 11 by J.A. Callow, H.W. Woolhouse (Eds.)

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